Lecture Advanced technologies in genomics for plant breeding (by Dutch Valley)
Tuesday, 24 September, 10:00 – 11:00
Language: English
1. Synchron, Glenn Dukel, BSc
Ultra-High Throughput automation of DNA extraction – the basis of genetic analysis
Most technologies for DNA analysis rely on good, clean DNA samples. Purifying DNA from breeding and QC material is a time consuming and cost significant part of the analysis process. In general, DNA extraction is performed by Liquid Handling robots with a parallel process, extracting several hundreds to thousands of samples per day. To significantly increase the throughput, Synchron has built a system that enables purifying tens of thousands of samples per day, using serial processing.
2. MolGen, Wim van Haeringen, PhD
Magnetic bead technology for DNA purification
MolGen’s mission is based on the never-ending necessity to provide our agricultural customers with validated, high-quality products to meet their growing demand for throughput and desire to lower the price per DNA extraction & purification. MolGen is ideally suited to take a multi-dimensional approach to help improve the solutions for your DNA extractions and purifications on e.g. Kingfisher, Oktopure and the DNA Cruiser. The presentation will show examples of optimization results on e.g. Oktopure
3. VHL genetics, Jon Wittendorp, BSc
DNA purification, singleplex and array-nased SNP genotyping and Next Generation Sequencing (NGS) technologies – which one to use when
Different downstream applications require different quality of DNA and DNA isolations. Also the use of genotyping platforms highly depends on the question that needs to be answered by the end user. The presentation will give a general overview on DNA quality/quantity and subsequent downstream genotyping applications.
4. Nimagen, Walter van der Vliet, BSc
RC-PCR and smMIP technologies for amplicon-based multiplex-PCR sample prep for Next Generation Sequencing (NGS) analysis
Genotyping your crops via targeted Next Generation Sequencing. Nimagen wants to show 2 approaches that allow for high throughput analysis and provides your genotype and extra sequence information. RC-PCR a single tube low multiplexing assay and smMIP is intended for high multiplexing, both technologies can be applied on high sample numbers per run.
5. Ugentec, Tom Martens, PhD
Using Artificial Intelligence (AI) and machine learning for automated real-time and end-point PCR analysis
PCR has proven to be an excellent technology to improve marker assisted breeding and detect pathogens in seeds and plants. With the increase in throughput, data analysis challenges arise. Data is not analyzed in a standardized fashion, errors are made during data transfer and employee churn increases due to boring, repetitive work. UgenTec’s solution FastFinder Analysis and FastFinder Genotyper offer robust data analysis solutions for respectively real-time PCR and end-point PCR, using artificial intelligence and data science technology to standardize data analysis while increasing throughput.
The goal of this workshop is to be able to give an overview of different technologies and automation being used by scientists in the plant breeding process. The talks are focused on introducing the different technologies, results and applicability in automated processes.